Polar bodies allow only the analysis of chromosomal disorders of the maternal genome and the analysis of meiosis I and II errors. This method can be combined with a biopsy of a blastomere on day 3 of the development.
The advantages of the method are:
- Enough time for genetic diagnosis;
- An embryo transfer in the same cycle;
- A minimal risk of embryo damage;
- can be conducted in countries where embryo diagnosis is prohibited (Germany, Austria).
The limitations of the method are:
- It reflects neither post-zygotic aneuploidies nor paternal aneuploidies;
- It cannot determine an embryo's gender;
- A PB biopsy cannot be repeated;
- Consecutive biopsies of the 1st PB in 4 hours after follicular aspiration and of the 2nd PB in 16-18 hours after insemination are strenuous and induce stress in an oocyte/zygote;
- A high price for genetic diagnosis (10 oocytes = 20 samples).