The ADVANTAGES are:
- The method allows the analysis not only of meiotic errors of the paternal and the maternal genome but also of mitotic ones;
- Enough time for genetic diagnosis;
- An embryo transfer in the same cycle;
- The biopsy can be repeated.
The LIMITATIONS are:
- Earlier studies claimed there was no harm for an embryo (Hardy et al., 1989), yet the modern ones speak about embryo damage (Mastenbroek et al, 2007, Scott et al, 2012);
- A decrease in the potential of implantation (Cohen et al, 2010);
- A high level of chromosomal mosaicism of pre-implantation embryos; a possibility that a healthy embryo can be diagnosed with a pathology, which nullifies the efficacy of diagnosis.
The consequences of a biopsy of blastomeres for an embryo:
- A decrease in the number of ICM and trophectoderm cells;
- A decrease in HCG-producing activity of a trophoblast when implanting embryos that have undergone a biopsy (Yeon Jean Cho et al, 2011).
Mice derived from embryos that had undergone a biopsy demonstrated:
- A change in the expression of 36 genes;
- Hypomielinization of nerve fibers in the brain;
- An increased risk of developing neurodegenerative diseases.